Perturbations of the hepatic proteome behind the onset of metabolic disorders in mouse offspring developed following embryo manipulation

Assisted Reproductive Technologies (ART) allowed the births of >8 million babies worldwide. Even if ART children are healthy at birth, several studies reported that may cause changes in foetal programming, leading to an increased predisposition metabolic disorders adulthood. Previous on mouse model showed obesity, glucose intolerance, and hepatic lipid accumulation offspring. A cumulative effect different components protocol has been previously described, for example, occurrence epigenetic defects. Here, we investigated whether there is a embryo transfer (ET), vitro culture (IVC) blastomere biopsy (BB) onset offspring vs those naturally conceived (Control - CTR). To this aim, proteomic analysis was performed livers from adult developed following ET, IVC BB CTR. We observed deregulated expression proteins involved lipid, carbohydrate, energy metabolisms cellular processes Moreover, found body weight all while i) insulin resistance male, ii) females intolerance high level triglycerides cholesterol iii) low levels interleukin-6 BB, ET males. In conclusion, our study suggests use various manipulations influences health offspring, resulting diseases syndrome sex-specific manner. • Embryo cumulatively increase risk Adaptations during early-development lead phenotypes manipulation perturbs proteome